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Isolation and identification of bacteria. (a) Growth of pure colonies of the six gut bacteria as follows: B1 and B2 in bacterial growth media, BHI1 and BHI2 in brain heart infusion, M1 and M2 in <t>MacConkey</t> media. (b) PCR amplification with 16S rRNA genes of the six isolates. The bands of high intensity correspond to positive results with positive bands at ~1500 bp of the six bacterial isolates (B1, B2, BHI1, BHI2, M1, M2). L: 1 kb DNA ladder, PC: positive control (16s rRNA fragments of Escherichia coli ATCC 8739), NC: negative control (nuclease free water).
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Isolation and identification of bacteria. (a) Growth of pure colonies of the six gut bacteria as follows: B1 and B2 in bacterial growth media, BHI1 and BHI2 in brain heart infusion, M1 and M2 in MacConkey media. (b) PCR amplification with 16S rRNA genes of the six isolates. The bands of high intensity correspond to positive results with positive bands at ~1500 bp of the six bacterial isolates (B1, B2, BHI1, BHI2, M1, M2). L: 1 kb DNA ladder, PC: positive control (16s rRNA fragments of Escherichia coli ATCC 8739), NC: negative control (nuclease free water).

Journal: Gut Microbes Reports

Article Title: The living medicine inside us: in vitro therapeutic prospects of human gut bacteria

doi: 10.1080/29933935.2025.2480093

Figure Lengend Snippet: Isolation and identification of bacteria. (a) Growth of pure colonies of the six gut bacteria as follows: B1 and B2 in bacterial growth media, BHI1 and BHI2 in brain heart infusion, M1 and M2 in MacConkey media. (b) PCR amplification with 16S rRNA genes of the six isolates. The bands of high intensity correspond to positive results with positive bands at ~1500 bp of the six bacterial isolates (B1, B2, BHI1, BHI2, M1, M2). L: 1 kb DNA ladder, PC: positive control (16s rRNA fragments of Escherichia coli ATCC 8739), NC: negative control (nuclease free water).

Article Snippet: These are Bacterial Growth Medium (BGM), Brain Heart Infusion (BHI) Medium (Condalab, Madrid, Spain) and MacConkey Agar Medium (Condalab, Madrid, Spain).

Techniques: Isolation, Bacteria, Amplification, Positive Control, Negative Control